Transcriptomics

Dataset Information

97

RNA-seq of C.elegans and M.musculus in the presence and absence of D-Glucosamine (GlcN)


ABSTRACT: D-Glucosamine (2-amino-2-deoxy-D-glucose, C.A.S.# 3416-24-8) (GlcN) is a freely available and commonly used dietary supplement possibly promoting cartilage health in humans which also acts as an inhibitor of glycolysis. We here find that GlcN extends C. elegans lifespan by impairing glucose metabolism to activate AMP-activated protein kinase (AMPK/AAK2) leading to increased mitochondrial biogenesis. Consistent with the concept of mitohormesis, this promotes increased formation of mitochondrial reactive oxygen species (ROS) and p38/PMK-1-mediated stress signaling culminating in increased expression of the nematodal amino acid-transporter 1 (aat-1) gene. Ameliorating mitochondrial ROS formation as well as impairment of aat-1-expression abolishes GlcN-mediated lifespan extension in a NRF2/SKN-1-dependent fashion. Notably and unlike other calorie restriction mimetics (CRM) like 2-deoxy-D-glucose (2DG, DOG), GlcN extends lifespan of aging C57BL/6 mice (log-rank: p=0.002; cox regression: p=0.01) similarly paralleled by an induction of mitochondrial biogenesis, increased expression of several murine amino acid transporters, as well as increased amino-acid catabolism. Taken together, GlcN mimics a ketogenic diet to extend healthspan in evolutionary distinct species. 24 samples: 12 mRNA profiles of C.elegans: 6 without GlcN and 6 with GlcN supplementaion; 12 mRNA profiles of M.musculus: 6 without GlcN and 6 with GlcN supplementaion

ORGANISM(S): Musculus  

SUBMITTER: Kim Zarse  Matthias Platzer   Doreen Kuhlow   Reinhard Guthke   Sandra Weimer   Tim J Schulz   Nicola Zamboni   Michael Ristow   Andreas F Pfeiffer   Troy L Merry   Steffen Priebe   Sébastien Dubuis   Johannes Mansfeld   Marco Groth   Josephine Priebs    

PROVIDER: E-GEOD-54853 | ArrayExpress | 2014-04-15

SECONDARY ACCESSION(S): SRP037555GSE54853PRJNA237825

REPOSITORIES: GEO, ArrayExpress, ENA

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