Dataset Information


VDR primary targets by genome-wide transcriptional profiling

ABSTRACT: We carried out a genome-wide investigation of the primary transcriptional targets of 1α,25(OH)2D3 in breast epithelial cancer cells using RNA-Seq technology. We identified early transcriptional targets of 1α,25(OH)2D3 involved in adhesion, growth regulation, angiogenesis, actin cytoskeleton regulation, hexose transport, inflammation and immunomodulation, apoptosis, endocytosis and signaling. Furthermore, we found several transcription factors to be regulated by 1α,25(OH)2D3 that subsequently amplify and diversify the transcriptional output driven by 1α,25(OH)2D3 leading finally to a growth arrest of the cells. Moreover, we could show that 1α,25(OH)2D3 elevates the trimethylation of histone H3 lysine 4 at several target gene promoters. Our present transcriptomic analysis of differential expression after 1α,25(OH)2D3 treatment provides a resource of primary 1α,25(OH)2D3 targets that might drive the antiproliferative action in breast cancer epithelial cells. ChIP-Seq for trimethylated histone H3K4 with SKBr3 cells treated for 2h with 100nM 1α,25(OH)2D3 or vehicle as control.

ORGANISM(S): Homo sapiens  

SUBMITTER: Giovanni Blandino   Francesca De Nicola  Sabrina Strano  Berardino Elmi  Paolo D’Onorio De Meo  Graziano Pesole  Matteo Pallocca  Frauke Goeman  Paola Muti  Maurizio Fanciulli  Tiziana Castrignanò 

PROVIDER: E-GEOD-54856 | ArrayExpress | 2014-06-25



Dataset's files

Action DRS
E-GEOD-54856.idf.txt Idf Processed
E-GEOD-54856.sdrf.txt Txt
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