Transcriptomics

Dataset Information

297

RNAseq of memory cocktail versus IL-2 grown OT-I CD8 T cells


ABSTRACT: Cytotoxic T cells are typically expanded ex vivo for adoptive immunotherapy by culture with IL-2. This culture period leads to a differentiated phenotype and acquisition of effector function, as well as a loss of in vivo proliferative capability and anti-tumor efficacy. Here, we report antigen-specific and polyclonal expansion of cytotoxic T cells in a cocktail of cytokines and small molecules that leads to a memory-like phenotype in mouse and human cells even during extended culture, leading to enhanced in vivo expansion and tumor control. OT-I CD8 T cells were cultured for 14 days in either IL-2 or a cocktail of memory inducing small molecules and cytokines (IL-7, IL-21, 2-deoxyglucose and TWS119). Populations were sorted, IL-2 cells were CD44+CD62L-, cocktail cells were sorted into CD44+CD62L-, CD44+CD62L+ and CD44lowCD62L+ populations, and naive OT-I cells were CD44-CD62L+. Total RNA was extracted from each population and prepared for sequencing as three technical replicates.

ORGANISM(S): Mus musculus  

SUBMITTER: David A Largaespada   Zohar Sachs  Rebecca LaRue  Adam J Litterman 

PROVIDER: E-GEOD-54877 | ArrayExpress | 2014-08-12

SECONDARY ACCESSION(S): SRP037709GSE54877PRJNA237954

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy.

Litterman Adam J AJ   Zellmer David M DM   LaRue Rebecca S RS   Jameson Stephen C SC   Largaespada David A DA  

Cancer immunology research 20140522 9


Cytotoxic T cells typically are expanded ex vivo in culture with IL2 for adoptive immunotherapy. This culture period leads to a differentiated phenotype and acquisition of effector function, as well as a loss of in vivo proliferative capability and antitumor efficacy. Here, we report antigen-specific and polyclonal expansion of cytotoxic T cells in a cocktail of cytokines and small molecules that leads to a memory-like phenotype in mouse and human cells even during extended culture, leading to e  ...[more]

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