Transcriptomics

Dataset Information

60

MicroRNA-15/16 Antagonizes c-Myb to Control Natural Killer Cell Maturation


ABSTRACT: NK cells develop in the bone marrow and complete their maturation in peripheral organs, but the molecular events controlling maturation are incompletely understood. Utilizing an NK cell-specific miR-15/16 deficient genetic model (15aKO), we identified a critical role for miR-15/16 family microRNAs in the normal maturation of NK cells in vivo, with a specific reduction in mature CD11b+CD27- NK cells in multiple tissues. The mechanism responsible was a block in differentiation, since accelerated NK cell death was not evident, and earlier intermediates of NK cell maturation were expanded. Further, we identified Myb as a direct target of miR-15/16 in NK cells, with Myb expression increased in immature 15aKO NK cells. Following adoptive transfer, immature 15aKO NK cells exhibited defective maturation, which was rescued by ectopic miR-15/16 expression or Myb knockdown. Moreover, Myb overexpression resulted in defective NK cell maturation. Thus, miR-15/16 regulation of Myb controls the normal NK cell maturation program. 3 technical replicates each of CD27+ 15a/16-1FKO NK cells, and CD27+ Ctrl NK cells

ORGANISM(S): Mus musculus  

SUBMITTER: Todd A Fehniger  Ryan P Sullivan    

PROVIDER: E-GEOD-55033 | ArrayExpress| 2015-09-04

SECONDARY ACCESSION(S): GSE55033PRJNA238299

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

MicroRNA-15/16 Antagonizes Myb To Control NK Cell Maturation.

Sullivan Ryan P RP   Leong Jeffrey W JW   Schneider Stephanie E SE   Ireland Aaron R AR   Berrien-Elliott Melissa M MM   Singh Anvita A   Schappe Timothy T   Jewell Brea A BA   Sexl Veronika V   Fehniger Todd A TA  

Journal of immunology (Baltimore, Md. : 1950) 20150812 6


NK cells develop in the bone marrow and complete their maturation in peripheral organs, but the molecular events controlling maturation are incompletely understood. The miR-15/16 family of microRNA regulates key cellular processes and is abundantly expressed in NK cells. In this study, we identify a critical role for miR-15/16 in the normal maturation of NK cells using a mouse model of NK-specific deletion, in which immature NK cells accumulate in the absence of miR-15/16. The transcription fact  ...[more]

Similar Datasets

| GSE58268 | GEO
2015-09-05 | E-GEOD-58268 | ArrayExpress
2015-07-08 | E-GEOD-50120 | ArrayExpress
2015-07-13 | E-GEOD-50120 | ExpressionAtlas
| GSE101470 | GEO
2011-12-31 | GSE30927 | GEO
2014-05-02 | E-GEOD-30927 | ArrayExpress
| PRJNA251855 | ENA
| PRJNA238299 | ENA
2011-06-10 | E-GEOD-13229 | ArrayExpress