Transcriptomics,Genomics

Dataset Information

298

Scml2 establshes germline-sepcific epigenome


ABSTRACT: we identify Scml2, a subunit of a germ cell-specific polycomb repressive complex 1 (PRC1), as a critical epigenetic modifier that establishes the germline-specific epigenome through two distinct functions. One of these functions is in the stem cell phase of spermatogonia and the other is on meiotic sex chromosomes. During the stem cell phase of spermatogonia, Scml2 establishes Rnf2- dependent ubiquitination of H2A (Rnf2-ubH2A) as an epigenetic memory that subsequently ensures programmed repression of somatic genes during the late stages of spermatogenesis. Additionally, during meiosis, Scml2 interacts with γH2AX and works downstream of the DNA damage response factor Mdc1 on the sex chromosomes and, contrary to autosomes, suppresses Rnf2-ubH2A for proper epigenetic programming of the sex chromosomes. Taken together, Scml2 positively regulates Rnf2-ubH2A on autosomes and negatively regulates Rnf2-ubH2A on the sex chromosomes to establish the germline-specific epigenome in spermatogenesis. Our study reveals a novel layer of epigenetic regulation in the male germline and adds further insight into the functionality of the polycomb proteins. RNA-seq and ChIP-seq analyses using wild-type and Scml2 KO spermatogenic cells

ORGANISM(S): Mus musculus  

SUBMITTER: Kazuteru Hasegawa   Satoshi Namekawa  Sin Hosu  Andrey V Kartashov  Artem Barski 

PROVIDER: E-GEOD-55060 | ArrayExpress | 2015-02-19

SECONDARY ACCESSION(S): GSE55060SRP037992PRJNA238350

REPOSITORIES: GEO, ArrayExpress, ENA

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