Dataset Information


Profiling hepatic microRNAs in zebrafish: Fluoxetine exposure mimics a fasting response that targets AMP-activated protein kinase (AMPK)

ABSTRACT: Recent evidence has suggested that fluoxetine, a serotonin-reuptake inhibitor and emerging environmental contaminant, can have non-targeted effects on metabolism in fish exposed to this waterborne pollutant. Using the highest, environmentally relevant, detectable level of fluoxetine (540 ng/L) we examined the impact of fluoxetine on the miRNA profile in the liver of zebrafish that were both fed and fasted for a period of 7 days. These results were further compared to the miRNA profile of zebrafish fasted and fed for 7 days, which were not exposed to fluoxetine. Results indicated that several miRNA that were involved with downregulating genes/pathways in response to fasting were also upregulated in fish exposed to fluoxetine, irrespective to fasting or feeding. These results suggest fluoxetine can have non-targeted effects on metabolic pathways mediated through miRNA expression. Furthermore, specific miRNA (dre-let-7d & dre-miR-140-5p) were found to target the catalytic subunit (AMPKa1 & AMPKa2, respectively) of AMP-Kinase, a master regulator of metabolism. Using predictive software and qPCR validation, combined with the expression profile of these two miRNA, we were able to establish a significant relationship between the expression of these specific miRNA to the downregulation of AMPKa subunit under the influence of 540 ng/L fluoxetine. Adult, female zebrafish were either fed or fasted for 7 days with and without the presense of 540 ng/L fluoxetine, and livers extracted and miRNA purified for miRNA microaary experiment.

ORGANISM(S): Danio rerio  

SUBMITTER: Paul Craig   Paul M Craig  Thomas W Moon  Vance L Trudeau 

PROVIDER: E-GEOD-55229 | ArrayExpress | 2014-02-21



Similar Datasets

| GSE31700 | GEO
2012-03-30 | E-GEOD-28955 | ArrayExpress
2012-02-23 | E-GEOD-31629 | ArrayExpress
2014-10-10 | E-GEOD-25820 | ExpressionAtlas
2012-06-30 | E-GEOD-35012 | ArrayExpress
2014-04-29 | E-GEOD-44692 | ArrayExpress
2010-10-12 | E-GEOD-18566 | ArrayExpress
2019-07-12 | PXD009086 | Pride
2009-05-20 | E-GEOD-16154 | ArrayExpress
2012-09-06 | E-GEOD-31712 | ArrayExpress