Transcriptomics

Dataset Information

3

Transcription profiling by array of Arabidopsis calcicole and calcifuge ecotypes after growth in high and low calcium conditions


ABSTRACT: The purpose of this set of arrays is to provide a third replicate to use in data analysis addition to the 2 previous replicates for all samples already generated on this project. Our hypothesis is that adaptation to a calcareous environment will be reflected in altered gene expression including genes encoding transporters, ion channels, transcription factors, etc. To test this hypothesis we grew a laboratory non-calcicole (Col-4) and a laboratory calcicole (Cal-0) ecotypes of A. thaliana at low (1 mM) and high (12.5 mM) rhizospheric Ca2+ and compare the patterns of gene expression by microarray analysis. We then collected from the wild, a putative calcicole ecotype (Elland)and a putative non-calcicole (Penicuik) and grew both at low (1 mM) and high (12.5 mM) rhizospheric Ca2+ and compared expression profiles to the laboratory ecotypes. Experimenter name = Bev Abram; Experimenter phone = 01524 592931; Experimenter address = Dept Biological Sciences; Experimenter address = Lancaster University; Experimenter address = Bailrigg; Experimenter address = Lancaster; Experimenter zip/postal_code = LA1 4YQ; Experimenter country = UK Experiment Overall Design: 8 samples were used in this experiment

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Nottingham Arabidopsis Stock Centre (NASC)  

PROVIDER: E-GEOD-5523 | ArrayExpress | 2008-06-13

SECONDARY ACCESSION(S): GSE5523PRJNA96179

REPOSITORIES: GEO, ArrayExpress

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