Genomics

Dataset Information

88

Identification of new regulators of the three dimensional Polycomb organization by a microscopy-based genome-wide RNAi screen


ABSTRACT: Polycomb group (PcG) proteins dynamically define cellular identities through epigenetic repression of key developmental genes. PcG target gene repression can be stabilized through the interaction in the nucleus at PcG foci. Here, we report the results of a high-resolution microscopy genome-wide RNAi screen that identifies 129 genes that regulate the nuclear organization of Pc foci. Candidate genes include PcG components and chromatin factors, as well as many novel protein-modifying enzymes, including components of the SUMOylation pathway. In the absence of SUMO Pc foci coagulate into larger aggregates. Conversely, loss of function of the SUMO peptidase velo disperses Pc foci. Moreover, SUMO and velo colocalize with PcG proteins at PREs and Pc SUMOylation affects its chromatin targeting, suggesting that the dynamic regulation of Pc SUMOylation regulates PcG-mediated silencing by modulating the kinetics of Pc binding to chromatin as well as its ability to form Polycomb foci. ChIP-Seq mapping of Polycomb (PC), SUMO and Velo on Drosophila Melanogaster

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Giacomo Cavalli  Julio Mateos-Langerak   Thierry Cheutin   Aubin Thomas   Inma Gonzalez    

PROVIDER: E-GEOD-55303 | ArrayExpress| 2014-05-06

SECONDARY ACCESSION(S): GSE55303SRP038890PRJNA239247

REPOSITORIES: GEO, ArrayExpress, ENA

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