Dataset Information


Transcription profiling by array of Arabidopsis knocked out for AtWRKY27

ABSTRACT: The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants. Experimenter name = Shahid Mukhtar; Experimenter phone = +49-221-5062-310; Experimenter fax = +49-221-5062-353; Experimenter address = Max.Planck-Institute for Plant Breeding; Experimenter address = Dept. Plant Microbe Interactions; Experimenter address = Carl-von-Linne Weg 10; Experimenter address = Koeln; Experimenter zip/postal_code = 50829; Experimenter country = Germany Experiment Overall Design: 4 samples were used in this experiment

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Nottingham Arabidopsis Stock Centre (NASC)   

PROVIDER: E-GEOD-5537 | ArrayExpress | 2008-06-13



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