Transcriptomics

Dataset Information

4

Transcription profile of SSEA-3-positive population of MSCs


ABSTRACT: Microarray analysis of the gene expression profile of SSEA-3-positive human adipose-derived MSCs were performed. Liposuction aspirates were obtained from the abdomen and thighs. Stromal vascular fraction (SVF) was isolated from the aspirated fat. Muse cells that express human SSEA-3 were collected from SVF using anti SSEA-3 antibody conjugated with PE and anti-PE microbeads. SSEA-3-negative fraction was considered ‘Muse-poor population’.

ORGANISM(S): Homo sapiens  

SUBMITTER: Kotaro Yoshimura   Hisako Ishimine  Hiromu Sugino  Kahori Kinoshita  Kentaro Doi  Noriyuki Aoi  Shinichiro Kuno  Akira Kurisaki  Kanako Watanabe-Susaki  Harunosuke Kato 

PROVIDER: E-GEOD-55526 | ArrayExpress | 2015-01-07

SECONDARY ACCESSION(S): GSE55526PRJNA239874

REPOSITORIES: GEO, ArrayExpress

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Publications

Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers.

Kinoshita Kahori K   Kuno Shinichiro S   Ishimine Hisako H   Aoi Noriyuki N   Mineda Kazuhide K   Kato Harunosuke H   Doi Kentaro K   Kanayama Koji K   Feng Jingwei J   Mashiko Takanobu T   Kurisaki Akira A   Yoshimura Kotaro K  

Stem cells translational medicine 20150105 2


Stage-specific embryonic antigen-3 (SSEA-3)-positive multipotent mesenchymal cells (multilineage differentiating stress-enduring [Muse] cells) were isolated from cultured human adipose tissue-derived stem/stromal cells (hASCs) and characterized, and their therapeutic potential for treating diabetic skin ulcers was evaluated. Cultured hASCs were separated using magnetic-activated cell sorting into positive and negative fractions, a SSEA-3+ cell-enriched fraction (Muse-rich) and the remaining frac  ...[more]

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