Transcriptomics,Genomics

Dataset Information

597

The Rhino-Deadlock-Cutoff complex licenses non-canonical transcription of dual-strand piRNA clusters in Drosophila


ABSTRACT: Argonaute proteins of the PIWI clade are central to transposon silencing in animal gonads. Their target specificity is defined by 22-30nt PIWI interacting RNAs (piRNAs), which mostly originate from discrete genomic loci termed piRNA clusters. Here we show that the RDC complex composed of Rhino, Deadlock and Cutoff defines dual-strand piRNA clusters genome-wide in Drosophila ovaries. The RDC complex is anchored to H3K9me3-marked chromatin in part via Rhino’s chromo-domain. Depletion of Piwi results in loss of the RDC and small RNAs at euchromatic piRNA source loci, demonstrating a feedback loop between Piwi and genomic piRNA sources. Intriguingly, profiles of RNA Polymerase II occupancy, nascent transcription and steady-state RNA levels reveal that the RDC licenses non-canonical transcription of dual-stranded piRNA clusters. Likely, this process involves 5’end protection of nascent RNAs and subsequent suppression of transcription termination. Together, our data provide a comprehensive model for the regulation and evolution of piRNA clusters. This study aims at indentifying and characterizing genimc sources of piRNA percursour transcripts using genome-wide apporaches such as ChIP-seq, RNA-seq, smallRNA-seq and GRO-seq in adult Drosophila ovaries depleted for several factors implicated in piRNA cluster regulation

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Dominik Handler   Grzegorz Sienski  Julius Brennecke  Fabio Mohn 

PROVIDER: E-GEOD-55824 | ArrayExpress | 2014-06-08

SECONDARY ACCESSION(S): SRP040008GSE55824PRJNA241078

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

The rhino-deadlock-cutoff complex licenses noncanonical transcription of dual-strand piRNA clusters in Drosophila.

Mohn Fabio F   Sienski Grzegorz G   Handler Dominik D   Brennecke Julius J  

Cell 20140601 6


Argonaute proteins of the PIWI clade are central to transposon silencing in animal gonads. Their target specificity is defined by 23-30 nt PIWI interacting RNAs (piRNAs), which mostly originate from discrete genomic loci termed piRNA clusters. Here, we show that a complex composed of Rhino, Deadlock, and Cutoff (RDC) defines dual-strand piRNA clusters genome-wide in Drosophila ovaries. The RDC is anchored to H3K9me3-marked chromatin in part via Rhino's chromodomain. Depletion of Piwi results in  ...[more]

Similar Datasets

2012-11-26 | E-GEOD-41729 | ArrayExpress
2015-08-31 | E-GEOD-71775 | ArrayExpress
2013-09-06 | E-GEOD-48317 | ArrayExpress
2015-06-07 | E-GEOD-64802 | ArrayExpress
2012-06-29 | E-GEOD-38728 | ArrayExpress
2013-05-10 | E-GEOD-45894 | ArrayExpress
2010-10-03 | E-GEOD-23560 | ArrayExpress
2013-08-01 | E-GEOD-47006 | ArrayExpress
2010-10-04 | GSE23560 | GEO
2011-02-10 | E-GEOD-26407 | ArrayExpress