Dataset Information


Gene expression analysis of eight-cell stage rat embryos sired by paternally exposed males to saline or BEP

ABSTRACT: 8-cell stage rat embryos were sired by males treated with saline or a chemotherapeutic cocktail, BEP, consisting of Bleomycin, Etoposide, and Cisplatin for 9 weeks. Animals were given an additional 9 weeks of recovery, without any treatment, prior to mating. qPCR gene expression profiling for 84 stem cell transcription factors. Total RNA extracted from 25-35 embryos at the eight-cell stage sired by control or BEP-treated males after a 9-wk recovery period (2 females mated per male, n= 4 males/treatment).

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Jennifer Maselli   Bernard Robaire  Barabara F Hales 

PROVIDER: E-GEOD-55842 | ArrayExpress | 2014-03-13



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Paternal exposure to testis cancer chemotherapeutics alters sperm fertilizing capacity and affects gene expression in the eight-cell stage rat embryo.

Maselli J J   Hales B F BF   Robaire B B  

Andrology 20140129 2

Treatment of testicular cancer includes the coadministration of bleomycin, etoposide and cis-platinum (BEP); however, along with its therapeutic benefit, BEP exposure results in extensive reproductive chemotoxic effects, including alterations to sperm chromatin integrity. As an intact paternal genome is essential for successful fertilization and embryogenesis, we assessed the effect of paternal exposure to BEP on sperm fertilization capacity and the resulting consequences on early embryonic gene  ...[more]

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