Transcriptomics

Dataset Information

299

Transcription profiling by high throughput sequencing of Arabidopsis siliques at four developmental stages


ABSTRACT: To search for the differential expressed genes during post-fertilization and embryonic development in Arabidopsis, We profiled transcriptome of siliques at 4 developmental stages using RNA-seq based on poly(A) selection. Each RNA library yielded 100 million 101-bp paired-end reads.Using Tophat and Cufflinks with the Arabidopsis TAIR10 annotation as reference, we identified 33,451 assembled transcripts in 4 samples. Of them, 5,608 were up-regulated genes in at least one sample. A group of them are preferentially expressed at mature green-stage of seeds. Transcriptome profiling in 0-5 day, 6-10 day, 11-15 day and 16-20 day post-anthesis siliques

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Nam-Hai Chua   Hui-Wen Wu  Jun Liu  Huan Wang 

PROVIDER: E-GEOD-55866 | ArrayExpress | 2016-03-28

SECONDARY ACCESSION(S): SRP040080GSE55866PRJNA241177

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis.

Liu Jun J   Deng Shulin S   Wang Huan H   Ye Jian J   Wu Hui-Wen HW   Sun Hai-Xi HX   Chua Nam-Hai NH  

Plant physiology 20160304 1


CURLY LEAF (CLF), a histone methyltransferase of Polycomb Repressive Complex 2 (PRC2) for trimethylation of histone H3 Lys 27 (H3K27me3), has been thought as a negative regulator controlling mainly postgermination growth in Arabidopsis (Arabidopsis thaliana). Approximately 14% to 29% of genic regions are decorated by H3K27me3 in the Arabidopsis genome; however, transcriptional repression activities of PRC2 on a majority of these regions remain unclear. Here, by analysis of transcriptome profiles  ...[more]

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