Transcriptomics

Dataset Information

3

Bovine Day 15 Extra-embryonic membrane (EEM): Male and Female Control vs. Male and Female CSF2 Methylation


ABSTRACT: We aimed to study the effects of CSF2 treatment on the methylome of the Day 15 female and male embryo. Methylomic profiling of bovine extra-embryonic membrane at Day 15 post-insemination produced by IVF. Oocytes were matured and fertilized in vitro using a single Holstein bull. After 5 days in vitro culture, the embryos were either treated with 10 ng/ml bovine recombinant CSF2 or the Control (DPBS/BSA) until Day 7. At Day 7, the embryos were transferred into recipients. At day 15 following insemination, embryos were flushed and collected for total RNA and gDNA extraction. The gender of the embryos were determined by PCR prior to microarray analysis. CSF2 males were compared to Control males while CSF2 females were compared to Control females

ORGANISM(S): Bos taurus  

SUBMITTER: D Gagne   Kyle Dobbs  C Robert  E Fournier  P J Hansen  M Sirard  K B Dobbs 

PROVIDER: E-GEOD-55873 | ArrayExpress | 2014-10-01

SECONDARY ACCESSION(S): GSE55873PRJNA241182

REPOSITORIES: GEO, ArrayExpress

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Publications

Sexual dimorphism in developmental programming of the bovine preimplantation embryo caused by colony-stimulating factor 2.

Dobbs Kyle B KB   Gagné Dominic D   Fournier Eric E   Dufort Isabelle I   Robert Claude C   Block Jeremy J   Sirard Marc-André MA   Bonilla Luciano L   Ealy Alan D AD   Loureiro Barbara B   Hansen Peter J PJ  

Biology of reproduction 20140730 3


Physiology of the adult can be modified by alterations in prenatal development driven by the maternal environment. Developmental programming, which can be established before the embryo implants in the uterus, can affect females differently than males. The mechanism by which sex-specific developmental programming is established is not known. Here we present evidence that maternal regulatory signals change female embryos differently than male embryos. In particular, actions of the maternally deriv  ...[more]

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