Transcriptomics

Dataset Information

69

Gene Expression Profiling of Prostate Benign and Malignant Tissue


ABSTRACT: We profiled genome-wide gene expression of human prostate benign and malignant tissue to identify potential biomarkers and immunotherapy targets. We stratified malignant specimens according to their TMPRSS2:ERG gene fusion status. Radical prostatectomy tissue samples were obtained from the Hershey Foundation Prostate Cancer Serum and Tumor Bank at our institution. Morphologic diagnosis was done by a pathologist. OCT blocks containing >30% of PCa tissue (with Gleason score of 6 or 7) were selected for RNA purification. A biopsy punch was used to select the PCa tissues from the OCT sample blocks. Benign or PCa tissues were homogenized using a TissueLyser (Qiagen) at 28 Hz for 5 min. Total RNA was isolated using Trizol reagent. RNA was quantified by NanoDrop ND-1000 spectrophotometer, and quality was evaluated with Agilent RNA 6000 NanoChip and the 2100 Bioanalyzer, with 28S/18S ratios and RIN determined by 2100 Expert software.

ORGANISM(S): Homo sapiens  

SUBMITTER: M Sanda   M S Arredouani  B Lu  M. Simo Arredouani 

PROVIDER: E-GEOD-55945 | ArrayExpress | 2014-03-18

SECONDARY ACCESSION(S): GSE55945PRJNA241405

REPOSITORIES: GEO, ArrayExpress

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Publications

Identification of the transcription factor single-minded homologue 2 as a potential biomarker and immunotherapy target in prostate cancer.

Arredouani Mohamed S MS   Lu Bin B   Bhasin Manoj M   Eljanne Miriam M   Yue Wen W   Mosquera Juan-Miguel JM   Bubley Glenn J GJ   Li Vivian V   Rubin Mark A MA   Libermann Towia A TA   Sanda Martin G MG  

Clinical cancer research : an official journal of the American Association for Cancer Research 20090908 18


Identification of novel biomarkers and immunotherapy targets for prostate cancer (PCa) is crucial to better diagnosis and therapy. We sought to identify novel PCa tumor-associated antigens (TAA) that are expressed in PCa, absent in nonprostate human tissue, and immunogenic for immune responses restricted by human HLA.Using microarray analysis of normal and cancerous human prostate tissues, we identified 1,063 genes overexpressed in PCa. After validating 195 transcripts in publicly available arra  ...[more]

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