Transcriptomics

Dataset Information

7

Differentiated human stem cells resemble fetal, not adult, β cells


ABSTRACT: Human pluripotent stem cells (hPSCs) have the potential to generate any human cell type, and one widely recognized goal is to make pancreatic β cells. To this end, comparisons between differentiated cell types produced in vitro and their in vivo counterparts are essential to validate hPSC-derived cells. Genome-wide transcriptional analysis of sorted insulin-expressing (INS(+)) cells derived from three independent hPSC lines, human fetal pancreata, and adult human islets points to two major conclusions: (i) Different hPSC lines produce highly similar INS(+) cells and (ii) hPSC-derived INS(+) (hPSC-INS(+)) cells more closely resemble human fetal β cells than adult β cells. This study provides a direct comparison of transcriptional programs between pure hPSC-INS(+) cells and true β cells and provides a catalog of genes whose manipulation may convert hPSC-INS(+) cells into functional β cells RNA is isolated and processed using MARIS from the following samples: H1 human embryonic stem cells (hESCs) in duplicate, HUES8 hESCs in duplicate, human induced pluripotent stem cells (hiPSCs) in duplicate, H1 cells differentiated to a stage in which insulin-expressing cells are present (stage 6) in duplicate, HUES8 cells differentiated to stage 6 in duplicate, hiPSCs differentiated to stage 6, insulin-expressing cells sorted from H1 cells differentiated to stage 6 in duplicate, insulin-expressing cells sorted from HUES8 cells differentiated to stage 6 in duplicate, insulin-expressing cells sorted from hiPSCs differentiated to stage 6 in duplicate, human week 16 fetal pancreata in duplicate, insulin-expressing cells sorted from human week 16 fetal pancreata in triplicate, adult human pancreatic islets in triplicate, and insulin-expressing cells sorted from adult human pancreatic islets in triplicate.

ORGANISM(S): Homo sapiens  

SUBMITTER: David K Gifford   Jeffrey R Millman  Siniša Hrvatin  Felicia W Pagliuca  Charles W O'Donnell  Douglas A Melton  Philip DiIorio  Sinisa Hrvatin  Alireza Rezania  Francis Deng 

PROVIDER: E-GEOD-56130 | ArrayExpress | 2014-03-25

SECONDARY ACCESSION(S): GSE56130PRJNA242526

REPOSITORIES: GEO, ArrayExpress

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Differentiated human stem cells resemble fetal, not adult, β cells.

Hrvatin Sinisa S   O'Donnell Charles W CW   Deng Francis F   Millman Jeffrey R JR   Pagliuca Felicia Walton FW   DiIorio Philip P   Rezania Alireza A   Gifford David K DK   Melton Douglas A DA  

Proceedings of the National Academy of Sciences of the United States of America 20140210 8


Human pluripotent stem cells (hPSCs) have the potential to generate any human cell type, and one widely recognized goal is to make pancreatic β cells. To this end, comparisons between differentiated cell types produced in vitro and their in vivo counterparts are essential to validate hPSC-derived cells. Genome-wide transcriptional analysis of sorted insulin-expressing (INS(+)) cells derived from three independent hPSC lines, human fetal pancreata, and adult human islets points to two major concl  ...[more]

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