Transcriptomics

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Transcription profiling by high throughput sequencing of cerebral cortex after cortical stroke in humans


ABSTRACT: It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA-rearrangements in the human neocortex. We show here that neither is the case, using immunohistochemistry, transcriptome-, genome- and ploidy-analyses, and determination of nuclear bomb test-derived 14C-concentration in neuronal DNA. A large proportion of cortical neurons display DNA-fragmentation and DNA-repair short time after stroke, whereas neurons at chronic stages after stroke show DNA-integrity, demonstrating the relevance of an intact genome for survival. Analyze of potential fusion transcripts in 13 samples, seven cortical ischemic stroke tissue and six control cortex, by deep sequencing using Illumina HiSeq 2000

REANALYSED by: E-GEOD-56267

ORGANISM(S): Homo sapiens  

SUBMITTER: Attila Rácz   Olaf Bergmann  Elisabet Englund  Emma Lindgren  Samuel Bernard  Zaal Kokaia  László Csiba  Andreas Dahl  Lena Ström  Tamás Csonka  Sofia Zdunek  Mehran Salehpour  Gábor Méhes  Tibor Hortobágyi  Stefan Schwab  Göran Possnert  Hagen B Huttner  Olle Lindvall  Benjamin Sigurgeirsson  Jemal Tatarishvili  Christian Scharenberg  Jonas Frisén  Joakim Lundeberg  Patrik Ståhl  Beata W Solnestam 

PROVIDER: E-GEOD-56267 | ArrayExpress | 2014-03-28

SECONDARY ACCESSION(S): GSE56267SRP040622PRJNA242801

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test-derived (14)C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating  ...[more]

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