Transcriptomics,Multiomics

Dataset Information

113

Transcription profiling by high throughput sequencing of cerebral cortex after cortical stroke in humans


ABSTRACT: It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA-rearrangements in the human neocortex. We show here that neither is the case, using immunohistochemistry, transcriptome-, genome- and ploidy-analyses, and determination of nuclear bomb test-derived 14C-concentration in neuronal DNA. A large proportion of cortical neurons display DNA-fragmentation and DNA-repair short time after stroke, whereas neurons at chronic stages after stroke show DNA-integrity, demonstrating the relevance of an intact genome for survival. Analyze of potential fusion transcripts in 13 samples, seven cortical ischemic stroke tissue and six control cortex, by deep sequencing using Illumina HiSeq 2000

REANALYSED by: E-GEOD-56267

ORGANISM(S): Homo sapiens  

SUBMITTER: Attila Rácz  Olaf Bergmann   Elisabet Englund   Emma Lindgren   Samuel Bernard   Zaal Kokaia   László Csiba   Andreas Dahl   Lena Ström   Tamás Csonka   Sofia Zdunek   Mehran Salehpour   Gábor Méhes   Tibor Hortobágyi   Stefan Schwab   Göran Possnert   Hagen B Huttner   Olle Lindvall   Benjamin Sigurgeirsson   Jemal Tatarishvili   Christian Scharenberg   Jonas Frisén   Joakim Lundeberg   Patrik Ståhl   Beata W Solnestam    

PROVIDER: E-GEOD-56267 | ArrayExpress | 2016-02-26

SECONDARY ACCESSION(S): GSE56267SRP040622PRJNA242801

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test-derived (14)C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating  ...[more]

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