Dataset Information


Transcriptional landscape of Rag2 -/- thymocytes [Mnase-Seq]

ABSTRACT: We performed ChIP-Seq for hallmark TFs (Ets1, Runx1), histone modification marks (H3K4me1, H3K4me2, H3K4me3, H3K27me3, H3K36me3), total RNA Pol II, short RNA-Seq as well as nucleosome mapping mainly in murine Rag2 -/- thymocytes. We also performed ChIP-Seq for E47 as well as nucleosome mapping, gene expression microarray analysis in CD4+ CD8+ DP thymocytes. Overall, we find a key role for the transcription factor Ets1, contributing towards alpha beta T cell lineage commitment via differential transactivation of stage-specific genes orchestrated by dynamic, co-association -mediated chromatin remodeling, as well as transcription dependent generation of a specialized chromatin structure at the TCR beta locus. Genome-wide analysis via ChIP-Seq for Ets1, Runx1, total RNA Pol II binding, H3K4me1, H3K4me2, H3K4me3, H3K27me3, H3K36me3, short RNA-Seq, Mnase-Seq in murine Rag2 -/- thymocytes, ChIP-Seq for E47, Mnase-Seq and gene expression microarray analysis in DP thymocytes This Series represents Mnase-Seq data.

ORGANISM(S): Mus musculus  

SUBMITTER: Salvatore Spicuglia  Angelica Santana   Ahmad Maqbool   Jean-Christophe Andrau   Joaquin Zacarias-Cabeza   Pierre Daniel Cauchy   Hélène Holota   Jean Imbert   Aleksandra Pekowska   Romain Fenouil   Pierre Ferrier   Laurent Vanhille   Frederic Koch   Pierre Cauchy   Aurélien Griffon    

PROVIDER: E-GEOD-56360 | ArrayExpress | 2014-05-06



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