Transcriptomics

Dataset Information

93

MicroRNA expression profiling of the fifth-instar posterior silk gland of Bombyx mori


ABSTRACT: Background: The growth and development of the posterior silk gland and the biosynthesis of the silk core protein at the fifth larval instar stage of Bombyx mori are of paramount importance for silk production. Results: Here, aided by next-generation sequencing and microarry assay, we profile 1,229 microRNAs (miRNAs), including 728 novel miRNAs and 110 miRNA/miRNA* duplexes, from the posterior silk gland at the fifth larval instar. Target gene prediction yields 14,222 unique target genes from 1,195 miRNAs. Functional categorization classifies the genes into complex pathways that include both cellular and metabolic processes, especially protein synthesis and processing. Conclusion: The enrichment of target genes in the ribosome-related pathway indicates that miRNAs may directly regulate translation. Our findings pave a way for further functional elucidation of these miRNAs in silk production. Sequencing 10 total RNA samples from the posterior silk gland of different strains and developmental stage using Illumina Solexa technology. Four strains of silkworm (Q, B, QB and BQ) with different two development stages (stage 1: fourth instar molting to day 2 of fifth instar; stage 2: fifth instar day 3 to day 8 before spinning, according to our previous genes expression cluster analysis), and two strains (R1 and J1) from entire period (stage 1 + stage 2).

ORGANISM(S): Bombyx mori  

SUBMITTER: Jun Yu  Jisheng Li   Yimei Cai   Zhengying You   Shaohua Wang   Boxiong Zhong   Jiaqian Che   Lupeng Ye    

PROVIDER: E-GEOD-56380 | ArrayExpress| 2014-09-06

SECONDARY ACCESSION(S): GSE56380SRP040738PRJNA243072

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

MicroRNA expression profiling of the fifth-instar posterior silk gland of Bombyx mori.

Li Jisheng J   Cai Yimei Y   Ye Lupeng L   Wang Shaohua S   Che Jiaqian J   You Zhengying Z   Yu Jun J   Zhong Boxiong B  

BMC genomics 20140529


BACKGROUND: The growth and development of the posterior silk gland and the biosynthesis of the silk core protein at the fifth larval instar stage of Bombyx mori are of paramount importance for silk production. RESULTS: Here, aided by next-generation sequencing and microarry assay, we profile 1,229 microRNAs (miRNAs), including 728 novel miRNAs and 110 miRNA/miRNA* duplexes, of the posterior silk gland at the fifth larval instar. Target gene prediction yields 14,222 unique target genes from 1,195  ...[more]

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