Transcriptomics

Dataset Information

92

Genome-wide mapping of yeast RNA polymerase II termination


ABSTRACT: Using a modification of the yeast anchor away and PAR-CLIP protocols, we have mapped the position of Pol II genome-wide in living yeast cells after depletion of components of either the polyadenylation (pA) or non-pA termination complexes. Depletion of Ysh1 from the nucleus does not lead to readthrough transcription. In contrast, depletion of the termination factor Nrd1 leads to widespread runaway elongation of non-pA transcripts. Depletion of Sen1 also leads to readthrough at non-pA terminators but in contrast to Nrd1 this readthrough appears less processive. Examination of PAR-CLIP sequencing data of Pol II after removal of different transcriptional termination factors.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Jeff Corden  Paul Schaughency   Jonathan Merran   Jeffry Corden    

PROVIDER: E-GEOD-56435 | ArrayExpress| 2014-09-06

SECONDARY ACCESSION(S): SRP040778GSE56435PRJNA243299

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2015-08-19 | E-GEOD-67483 | ArrayExpress
2013-11-13 | E-MTAB-1766 | ArrayExpress
2014-06-26 | E-GEOD-40254 | ArrayExpress
2015-08-19 | E-GEOD-57155 | ArrayExpress
2011-06-01 | GSE19015 | GEO
| GSE79222 | GEO
2014-05-02 | E-GEOD-19015 | ArrayExpress
2014-05-03 | E-MTAB-1060 | ArrayExpress
2013-08-22 | E-GEOD-31764 | ArrayExpress
| GSE97345 | GEO