Transcriptomics

Dataset Information

6

Expression data from human monocyte derived macrophages infected with adenovirus expressing HIV-1 Vpr


ABSTRACT: HIV-1 Vpr protein is a multifunctional protein which perturbs human transcriptome and interacts with a number of cellular proteins. In this study, we have attempted to explore the efffects of Vpr on human transcriptome and have identified several genes which are involved in innate immune respone and cell signaling pathways. We used the microarray analysis to elucidate the differnetail expression pattern of differnet genes in human macrophages infected with HIV-1 Vpr. As result we found that HIV-1 Vpr protein leads to the induction of various interferon stimualted genes (ISGs) and chemokines in human macrophages. Human monocytes-derived macrophages (MDMs) were isolated from peripheral blood mononuclear cells (PBMCs) from two healthy donors and were infected with recombinant adenoviruses either expressing HIV-1 Vpr or ZsGreen1 as a control. At 48 hours post-infection, RNA was isolated and subjected to microarray analysis.

ORGANISM(S): Homo sapiens  

SUBMITTER: Guangai Xue   Aida Yoko  Yoko Aida  Muhammad A Zahoor  Keisuke Fukumoto 

PROVIDER: E-GEOD-56591 | ArrayExpress | 2014-04-09

SECONDARY ACCESSION(S): GSE56591PRJNA244012

REPOSITORIES: GEO, ArrayExpress

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