Transcriptomics

Dataset Information

2

Contrast of poly-ribosomal mRNA in germina vesicle (GV) and metaphase II (MII) bovine oocytes.


ABSTRACT: Poly-ribosomal mRNA from pools of 75 bovine oocytes at the germinal vesicle(GV) and metaphase II(MII) stages were isolated by sucrose fractionation then compared through microarray hybridization. 3 biological replicates from germinal vesicle(GV) and metaphase II(MII) bovine oocytes were hybridized against each other while alternating dyes. One additional technical replicate (dye-swap of one of the previous hybridizations) was added to balance dye usage. The normalized log2 ratio (GV/MII) data is provided in the 'normalized_data.txt' file. Please note that data were scanned/extracted using a different Agilent reference file, and thus the feature extraction number for a given probe (in both normalized and raw data files) is different from that in the official Agilent-041017 platform (GPL18537). The reference file used for this study is provided as a Series supplementary file (041017_D_DNAFront_BCBottom_20120517.txt).

ORGANISM(S): Bos taurus  

SUBMITTER: Eric Fournier   Claude Robert  Angus Macaulay  Sara Scantland  Carolina H Macabelli  Alberto M Luciano  Irene Tessaro  Gaël Cagnone 

PROVIDER: E-GEOD-56603 | ArrayExpress | 2014-09-09

SECONDARY ACCESSION(S): GSE56603PRJNA244050

REPOSITORIES: GEO, ArrayExpress

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