Dataset Information


Expression profiling of EvgSc and ompR mutants

ABSTRACT: In order to explore the transcriptional reprogramming in EvgSc, ompR and EvgSc/ompR mutants we have used whole genome microarray profiling. EvgSc, ompR and EvgSc/ompR mutants were developed. Each mutant was profiled in duplicate.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655  

SUBMITTER: Kim Clarke   Pete Lund 

PROVIDER: E-GEOD-56833 | ArrayExpress | 2015-05-20



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Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance in Escherichia coli.

Johnson Matthew D MD   Bell James J   Clarke Kim K   Chandler Rachel R   Pathak Prachi P   Xia Yandong Y   Marshall Robert L RL   Weinstock George M GM   Loman Nicholas J NJ   Winn Peter J PJ   Lund Peter A PA  

Molecular microbiology 20140724 5

Laboratory-based evolution and whole-genome sequencing can link genotype and phenotype. We used evolution of acid resistance in exponential phase Escherichia coli to study resistance to a lethal stress. Iterative selection at pH 2.5 generated five populations that were resistant to low pH in early exponential phase. Genome sequencing revealed multiple mutations, but the only gene mutated in all strains was evgS, part of a two-component system that has already been implicated in acid resistance.  ...[more]

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