Dataset Information


Transcription profiling by array of Arabidopsis after infection with Botrytis cinerea

ABSTRACT: AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana; The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Responses to B. cinerea infection were assayed in wild-type Col-0 plants at 18 and 48 hours after inoculation of adult leaves. Leaves were inoculated by placing 4 5-ul drops of a 5x10e5 spore solution on each leaf. Control leaves were spotted with droplets of 24g L-1 potato dextrose broth medium. Experimenter name = Carine Denoux , Fred Ausubel , Julia Dewdney , Simone Ferrari; Experimenter institute = AtGenExpress Experiment Overall Design: 12 samples were used in this experiment

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Nottingham Arabidopsis Stock Centre (NASC)  

PROVIDER: E-GEOD-5684 | ArrayExpress | 2008-06-14



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