Transcriptomics,Genomics

Dataset Information

307

Transcription factor cooperativity in early adipogenic hotspots and super-enhancers


ABSTRACT: Here we have used a combination of advanced proteomics and genomics approaches to investigate the extent and mechanisms of transcription factor cross-talk at genomic hotspots. We identify ~12,000 transcription factor hotspots in the early phase of adipogenesis, and we find evidence of both simultaneous and sequential binding of transcription factors at these regions. We demonstrate for the first time that hotspots are highly enriched in large super-enhancer regions and that these drive the early adipogenic reprogramming of gene expression. Our results indicate that cooperativity between transcription factors at the level of hotspots as well as super-enhancers is very important for enhancer activity and transcriptional reprogramming. Thus, hotspots and super-enhancers constitute important regulatory hubs integrating external stimuli on chromatin. Genome-wide profiling of transcription factor and co-factor binding, epigenomic marks, and gene expression in 3T3-L1 pre-adipocytes.

ORGANISM(S): Mus musculus  

SUBMITTER: Susanne Mandrup   Simone Sidoli  Lars L Poulsen  Adelina Rogowska-Wrzesinska  Anne Loft  Ole N Jensen  Rasmus Siersbæk  Ronni Nielsen  Sofie Traynor  Atefeh Rabiee 

PROVIDER: E-GEOD-56872 | ArrayExpress | 2014-04-17

SECONDARY ACCESSION(S): GSE56872SRP041249PRJNA244848

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


It is becoming increasingly clear that transcription factors operate in complex networks through thousands of genomic binding sites, many of which bind several transcription factors. However, the extent and mechanisms of crosstalk between transcription factors at these hotspots remain unclear. Using a combination of advanced proteomics and genomics approaches, we identify ∼12,000 transcription factor hotspots (∼400 bp) in the early phase of adipogenesis, and we find evidence of both simultaneous  ...[more]

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