Transcriptomics

Dataset Information

591

4sUDRB-seq: measuring transcription elongation and initiation genomewide


ABSTRACT: A new method to measure elongation and intitiation rates Reversal inhibition of transcription with DRB and tagging newly transcribed RNA with 4-thiouridine (4sU)

REANALYSED by: E-GEOD-57116

ORGANISM(S): Homo sapiens  

SUBMITTER: Gilad Fuchs   Yoav Voichek 

PROVIDER: E-GEOD-57116 | ArrayExpress | 2014-05-09

SECONDARY ACCESSION(S): GSE57116SRP041471PRJNA245468

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

4sUDRB-seq: measuring genomewide transcriptional elongation rates and initiation frequencies within cells.

Fuchs Gilad G   Voichek Yoav Y   Benjamin Sima S   Gilad Shlomit S   Amit Ido I   Oren Moshe M  

Genome biology 20140509 5


Although transcriptional elongation by RNA polymerase II is coupled with many RNA-related processes, genomewide elongation rates remain unknown. We describe a method, called 4sUDRB-seq, based on reversible inhibition of transcription elongation coupled with tagging newly transcribed RNA with 4-thiouridine and high throughput sequencing to measure simultaneously with high confidence genome-wide transcription elongation rates in cells. We find that most genes are transcribed at about 3.5 Kb/min, w  ...[more]

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