Transcriptomics

Dataset Information

298

The role of carbon starvation in the induction of enzymes that degrade plant-derived carbohydrates in Aspergillus niger


ABSTRACT: Fungi are an important source of enzymes for saccharification of plant polysaccharides and production of biofuels. Understanding of the regulation and induction of expression of genes encoding these enzymes is still incomplete. To explore the induction mechanism, we analysed the response of the industrially important fungus Aspergillus niger to wheat straw, with a focus on events occurring shortly after exposure to the substrate. RNA sequencing showed that over a third of the genes induced after 6 h of exposure to wheat straw were also induced during 6 h of carbon starvation, indicating that carbon starvation is probably an important factor in the early response to wheat straw. The up-regulation of the expression of a high number of genes encoding CAZymes that are active on plant-derived carbohydrates during early carbon starvation suggests that these enzymes could be involved in a scouting role during starvation, releasing inducing sugars from complex plant polysaccharides. Eight samples in total consisting of duplicate shake flask Aspergillus niger cultures from four conditions: 48h glucose, 6 h starvation, 6 h wheat straw, 24 h starvation

ORGANISM(S): Aspergillus niger  

SUBMITTER: Stephane Delmas   Emelie C Noltorp  Richard Fetherston  Sunir Malla  Kristin Wennberg  Jolanda M van Munster  Xiaolan Yu  Paul Dupree  Matthew Kokolski  Martin J Blythe  David Archer  Richard G Beniston  David B Archer  Steven T Pullan  Paul Daly 

PROVIDER: E-GEOD-57315 | ArrayExpress | 2014-08-22

SECONDARY ACCESSION(S): GSE57315SRP041680PRJNA246235

REPOSITORIES: GEO, ArrayExpress, ENA

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The role of carbon starvation in the induction of enzymes that degrade plant-derived carbohydrates in Aspergillus niger.

van Munster Jolanda M JM   Daly Paul P   Delmas Stéphane S   Pullan Steven T ST   Blythe Martin J MJ   Malla Sunir S   Kokolski Matthew M   Noltorp Emelie C M ECM   Wennberg Kristin K   Fetherston Richard R   Beniston Richard R   Yu Xiaolan X   Dupree Paul P   Archer David B DB  

Fungal genetics and biology : FG & B 20140429


Fungi are an important source of enzymes for saccharification of plant polysaccharides and production of biofuels. Understanding of the regulation and induction of expression of genes encoding these enzymes is still incomplete. To explore the induction mechanism, we analysed the response of the industrially important fungus Aspergillus niger to wheat straw, with a focus on events occurring shortly after exposure to the substrate. RNA sequencing showed that the transcriptional response after 6h o  ...[more]

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