Genomics

Dataset Information

67

Genomic profiling of DNA methyltransferases reveals a role for DNMT3B in genic methylation


ABSTRACT: This SuperSeries is composed of the SubSeries listed below. DNA methylation is an epigenetic modification associated with transcriptional repression of promoters and is essential for mammalian development. Establishment of DNA methylation is mediated by the de novo DNA methyltransferases DNMT3A and DNMT3B, whereas DNMT1 ensures maintenance of methylation through replication. Absence of these enzymes is lethal, and somatic mutations in these genes have been associated with several human diseases. How genomic DNA methylation patterns are regulated remains poorly understood, as the mechanisms that guide recruitment and activity of DNMTs in vivo are largely unknown. To gain insights into this matter we determined chromosomal binding and site-specific activity of the mammalian de novo DNA methyltransferases DNMT3A and DNMT3B. We show that both enzymes localize to methylated, CpG dense regions in mouse stem cells, yet are excluded from active promoters and enhancers. By specifically measuring sites of de novo methylation, we observe that enzymatic activity reflects chromosomal binding. De novo methylation increases with CpG density, yet is excluded from nucleosomes. Notably, we observed selective binding of DNMT3B to the bodies of transcribed genes, which leads to their preferential methylation. This targeting to transcribed sequences requires SETD2-mediated methylation of lysine 36 on histone H3 and a functional PWWP domain of DNMT3B. Together these findings reveal how sequence and chromatin cues guide de novo methyltransferase activity to ensure methylome integrity. Refer to individual Series

ORGANISM(S): Mus musculus  

SUBMITTER: Dirk Schuebeler   

PROVIDER: E-GEOD-57413 | ArrayExpress | 2015-01-27

SECONDARY ACCESSION(S): GSE57413PRJNA246408

REPOSITORIES: GEO, ArrayExpress

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Publications

Genomic profiling of DNA methyltransferases reveals a role for DNMT3B in genic methylation.

Baubec Tuncay T   Colombo Daniele F DF   Wirbelauer Christiane C   Schmidt Juliane J   Burger Lukas L   Krebs Arnaud R AR   Akalin Altuna A   Schübeler Dirk D  

Nature 20150121 7546


DNA methylation is an epigenetic modification associated with transcriptional repression of promoters and is essential for mammalian development. Establishment of DNA methylation is mediated by the de novo DNA methyltransferases DNMT3A and DNMT3B, whereas DNMT1 ensures maintenance of methylation through replication. Absence of these enzymes is lethal, and somatic mutations in these genes have been associated with several human diseases. How genomic DNA methylation patterns are regulated remains  ...[more]

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