Dataset Information


MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

ABSTRACT: MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by Ago2 CrossLinking ImmunoPrecipitation coupled with sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1 mediated translational stimulation in the mitochondria and repression in the cytoplasm. Examination of miRNA's regulation function in mitochondria in C2C12 myoblasts cells and myotubes cells with CLIP-seq (Ago2).

ORGANISM(S): Mus musculus  

SUBMITTER: Huiqiong Zhang  Jie Zhou   Bo Yang   Yu Zhou   Yi Zhang   Xiang-Dong Fu   Jie Huang   Lin Guo   Xiaorong Zhang   Yuanchao Xue   Xiaolu Zhao   Hui Sun   Yun Yan   Peipei Guo   Xinxin Zuo   Zongran Li    

PROVIDER: E-GEOD-57596 | ArrayExpress | 2014-09-06



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