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Human XCR1+ Dendritic Cells Derived In Vitro from CD34+ Progenitors Closely Resemble Blood Dendritic Cells, Including Their Adjuvant Responsiveness, Contrary to Monocyte-Derived Dendritic Cells

ABSTRACT: Functional characterization of human dendritic cell subsets is limited due to the very low frequency of these cells in vivo. We developed an in vitro culture system for the simultaneous generation of XCR1+ DCs and MoDCs from cord blood CD34+ cells. Their global gene expression profiles at steady state and under activation, phenotypes, morphologies and responses to different TLR ligands where characterized and compared with those of their in vivo counterparts. The study demonstrated that the XCR1+ DCs generated in vitro from cord blood CD34+ cells are equivalent to blood XCR1+ DCs and also allowed a rigorous comparison of this DC subset with MoDC which are often considered as the reference model for DCs. Altogether, our results showed that in vitro generated XCR1+ DCs are a better model for the study of blood DC than the conventionally used MoDCs. The different dendritic cell subsets used for the study were either generated in vitro from cord blood CD34+ cells with recombinant human cytokines or isolated from peripheral blood. The subsets were purified by fluorescence-activated cell sorting and their responses to PolyI:C, LPS or R848 were studied including by gene expression profiling.


ORGANISM(S): Homo Sapiens

SUBMITTER: D Bechlian   E Fossum  R Chelbi  H Liu  A Doffin  C Caux  B Bogen  J Valladeau-Guilemond  C Sanchez  F Montanana-Sanchis  T Soos  C Asselin-Paturel  J Savoret  M Shaw  Marc Dalod  C Chabannon  I Perrot  M Dalod  V Ollion  S Balan  Tp Vu Manh  N Colletti 

PROVIDER: E-GEOD-57671 | ArrayExpress | 2014-07-23



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Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.

Balan Sreekumar S   Ollion Vincent V   Colletti Nicholas N   Chelbi Rabie R   Montanana-Sanchis Frédéric F   Liu Hong H   Vu Manh Thien-Phong TP   Sanchez Cindy C   Savoret Juliette J   Perrot Ivan I   Doffin Anne-Claire AC   Fossum Even E   Bechlian Didier D   Chabannon Christian C   Bogen Bjarne B   Asselin-Paturel Carine C   Shaw Michael M   Soos Timothy T   Caux Christophe C   Valladeau-Guilemond Jenny J   Dalod Marc M  

Journal of immunology (Baltimore, Md. : 1950) 20140709 4

Human monocyte-derived dendritic cell (MoDC) have been used in the clinic with moderately encouraging results. Mouse XCR1(+) DC excel at cross-presentation, can be targeted in vivo to induce protective immunity, and share characteristics with XCR1(+) human DC. Assessment of the immunoactivation potential of XCR1(+) human DC is hindered by their paucity in vivo and by their lack of a well-defined in vitro counterpart. We report in this study a protocol generating both XCR1(+) and XCR1(-) human DC  ...[more]

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