Genomics

Dataset Information

240

Modeling Fanconi Anemia pathogenesis and therapeutics using integration-free patient iPSCs


ABSTRACT: To elucidate the transcriptional and epigenetic alterations underlying the neurogenic defects of FA-NSCs, we conducted gene expression microarray analysis and global DNA methylation profiling. The gene expression pattern of gene-corrected NSCs (C-FA-NSCs) resembled that of control-NSCs but clustered distantly from FA-NSCs (Fig. 6F and Table S1). Hierarchical clustering based on DNA methylation levels in the promoter region (+/-1.5kb from TSS) of genes whose expression levels were rescued in C-FA-NSCs, placed C-FA-NSCs closer to control-NSCs and away from FA-NSCs (Fig. 6G), although this pattern was not seen at the whole genome level (Fig. S4C). This suggests that FANCA gene correction leads to specific methylation changes in a subset of promoters. Examination of the methylomes of NSCs derived from Fanconi Anemia iPSCs before and after gene correction by targeted bisulfite sequencing with padlock probes

ORGANISM(S): Homo sapiens  

SUBMITTER: Juan P Trujillo   Jessica Kim  Xiuling Xu  Carolina Tarantino  Ying Li   Mo Li  April Goebl  Shigeo Masuda  Ying Gu  Fei Yi  Emmanuel Nivet  Jordi Surralles  Concepcion R Esteban  Emi Aizawa  Ilir Dubova  Chris Benner  Weiqi Zhang  Guang-Hui Liu  Carlo Dufour  Nongluk Plongthongkum  Antonio del Sol  Nuria Montserrat  Rupa Soligalia  Ruotong Ren  Na Y Kim  Juan Bueren  Kun Zhang  Sergio Ruiz  Enrico Cappelli  Keiichiro Suzuki  Yuji Tsunekawa  Juan C Belmonte  Jing Qu 

PROVIDER: E-GEOD-57685 | ArrayExpress | 2014-05-15

SECONDARY ACCESSION(S): GSE57685SRP041996PRJNA247678

REPOSITORIES: GEO, ArrayExpress, ENA

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