Transcriptomics

Dataset Information

300

The exon junction complex controls transposable element activity by ensuring the faithful splicing of the piwi transcript


ABSTRACT: The exon junction complex (EJC) is a highly conserved ribonucleoprotein complex which binds RNAs at a late stage of the splicing reaction and remains associated following export to the cytoplasm. This complex is involved in several cellular post-transcriptional processes including mRNA localization, translation and degradation. The EJC plays an additional role in the splicing of a subset of genes in Drosophila and in human cells but the underlying mechanism remains to be elucidated. Here, we have found a novel function for the EJC and its splicing subunit RnpS1 in preventing transposon accumulation in both Drosophila germline and surrounding follicular cells. This function is mediated specifically through the control of the splicing of the piwi transcript. In absence of RnpS1 one of the piwi intron is retained. This intron contains a weak 5’ splice site as well as degenerate transposon fragments, reminiscent of heterochromatic introns. In addition, we identified a small A/T rich region, which alters its polypyrimidine tract (PPT) and confers the RnpS1’s dependency. Finally, we showed that the removal of this intron by RnpS1 requires the initial splicing of the flanking introns, suggesting a model in which the EJC facilitates the splicing of challenging introns following its initial deposition to adjacent exon junctions. In total there are 4 different conditions. Comparisons were made between piwi mutant vs control piwi and rnps1 KD vs controls RnpS1

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Colin Malone   Junaid Akhtar  Jean-Yves Roignant  Nastasja Kreim 

PROVIDER: E-GEOD-57710 | ArrayExpress | 2014-09-08

SECONDARY ACCESSION(S): GSE57710SRP042020PRJNA247816

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript.

Malone Colin D CD   Mestdagh Claire C   Akhtar Junaid J   Kreim Nastasja N   Deinhard Pia P   Sachidanandam Ravi R   Treisman Jessica J   Roignant Jean-Yves JY  

Genes & development 20140807 16


The exon junction complex (EJC) is a highly conserved ribonucleoprotein complex that binds RNAs during splicing and remains associated with them following export to the cytoplasm. While the role of this complex in mRNA localization, translation, and degradation has been well characterized, its mechanism of action in splicing a subset of Drosophila and human transcripts remains to be elucidated. Here, we describe a novel function for the EJC and its splicing subunit, RnpS1, in preventing transpos  ...[more]

Similar Datasets

2014-08-14 | E-GEOD-59327 | ArrayExpress
2014-12-04 | E-GEOD-58830 | ArrayExpress
2011-12-01 | E-GEOD-32950 | ArrayExpress
2010-10-20 | GSE24012 | GEO
| GSE81460 | GEO
2014-11-04 | E-GEOD-33475 | ArrayExpress
2010-10-19 | E-GEOD-24012 | ArrayExpress
2013-02-04 | E-GEOD-34032 | ArrayExpress
2013-02-09 | E-GEOD-43360 | ArrayExpress
2013-05-10 | E-GEOD-46100 | ArrayExpress