Transcriptomics

Dataset Information

306

Transcription profiling by high throughput sequencing of three rice genotypes in response to drought stress


ABSTRACT: To dissect the molecular mechanisms underlying drought tolerance (DT) in rice, transcriptome differences of a DT introgression line H471, the DT donor P28 and the drought sensitive recurrent parent HHZ under drought stress were investigated using deep transcriptome sequencing. Results revealed a differential constitutive gene expression prior to stress and distinct global transcriptome reprogramming among three genotypes under time-series drought stress, consistent with their differential genotypes and DT phenotypes. DT introgression line H471, the DT donor P28 and the drought sensitive recurrent parent HHZ under drought stress were investigated using deep transcriptome sequencing.The drought stress treatment was started by withholding water at the tillering stage. The days were counted after the AWC in the soil reached 20% to allow drought measurements at precisely determined intervals, and the soil water content reached 15%, 10% and 7.5% after 1d, 3d and 4d drought treatment, respectively.Three top leaves for each sample were harvested for each genotype under 1d and 3d drought stress and control conditions. All samples were immediately frozen in liquid nitrogen and stored at -80C and then for transcriptome sequencing.

ORGANISM(S): Oryza sativa  

SUBMITTER: Binying Fu   Liyu Huang  Fan Zhang  Yongli Zhou 

PROVIDER: E-GEOD-57950 | ArrayExpress | 2014-12-30

SECONDARY ACCESSION(S): GSE57950SRP042233PRJNA248474

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Comparative transcriptome sequencing of tolerant rice introgression line and its parents in response to drought stress.

Huang Liyu L   Zhang Fan F   Zhang Fan F   Wang Wensheng W   Zhou Yongli Y   Fu Binying B   Li Zhikang Z  

BMC genomics 20141126


BACKGROUND: Rice (Oryza sativa. L) is more sensitive to drought stress than other cereals, and large genotypic variation in drought tolerance (DT) exists within the cultivated rice gene pool and its wild relatives. Selective introgression of DT donor segments into a drought-sensitive (DS) elite recurrent parent by backcrossing is an effective way to improve drought stress tolerance in rice. To dissect the molecular mechanisms underlying DT in rice, deep transcriptome sequencing was used to inves  ...[more]

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