Transcriptomics

Dataset Information

7

Microtubule Affinity-Regulating Kinase 2 is associated with DNA damage response and cisplatin resistance in non-small cell lung cancer.


ABSTRACT: MARK2 expression is associated with the malignant phenotype, expression of DNA repair components, NF-κB inhibition and cell cycle transcription factors in lung cancer cells We used microarrays to determine the global gene expression pattern associated with knockdown of MARK2 3 lung cancer cell lines overexpressing MARK2 have been selected to assess the gene expression pattern after MARK2 knockdown. RNA extraction and hybridization on Affymetrix microarrays have been done on 2 biological replicates for each line (H1993, H1693 and H1650) after shRNA lentiviral transduction and compared with respective contols using the empty vector pLKO.

ORGANISM(S): Homo sapiens  

SUBMITTER: Kelsie L Thu   Emily A Vucic  Larissa A Pikor  Stephen Lam  Wan L Lam  Adi F Gazdar  Victor D Martinez  Roland Hubaux  Daiana D Becker-Santos  Sonia H Kung  Mitra Mosslemi 

PROVIDER: E-GEOD-57966 | ArrayExpress | 2015-06-22

SECONDARY ACCESSION(S): GSE57966PRJNA248590

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Microtubule affinity-regulating kinase 2 is associated with DNA damage response and cisplatin resistance in non-small cell lung cancer.

Hubaux Roland R   Thu Kelsie L KL   Vucic Emily A EA   Pikor Larissa A LA   Kung Sonia H Y SH   Martinez Victor D VD   Mosslemi Mitra M   Becker-Santos Daiana D DD   Gazdar Adi F AF   Lam Stephen S   Lam Wan L WL  

International journal of cancer 20150506 9


Microtubule affinity-regulating kinases (MARKs) are involved in several cellular functions but few studies have correlated MARK kinase expression with cancer, and none have explored their role in lung cancer. In this study, we identified MARK2 as frequently disrupted by DNA hypomethylation and copy gain, resulting in concordant overexpression in independent lung tumor cohorts and we demonstrate a role for MARK2 in lung tumor biology. Manipulation of MARK2 in lung cell lines revealed its involvem  ...[more]

Similar Datasets

2016-09-30 | E-MTAB-4671 | ArrayExpress
| GSE69747 | GEO
2016-06-10 | E-GEOD-83161 | ArrayExpress
2014-06-12 | E-GEOD-58398 | ArrayExpress
2016-02-28 | E-GEOD-44766 | ArrayExpress
| GSE121217 | GEO
| GSE104963 | GEO
2013-03-19 | E-GEOD-38851 | ArrayExpress
2012-06-30 | E-GEOD-36821 | ArrayExpress
| GSE103346 | GEO