Genomics

Dataset Information

36

MacroH2A histone variants have a major role in nuclear organization and tether heterochromatinic repeats to lamin B1


ABSTRACT: Genetic loss-of-function studies in development, cancer and somatic cell reprogramming suggested that the group of macroH2A histone variants could function through stabilizing the differentiated state by a yet unknown mechanism. Here, we now present results demonstrating that macroH2A variants have a major function in maintaining nuclear organization and heterochromatin architecture. Specifically, we find that a substantial amount of macroH2A is associated with heterochromatic repeat sequences in an H3K9me3-dependent manner. We further identify macroH2A on sites of interstitial heterochromatin. Loss of macroH2A leads to major defects in nuclear organization including loss of nuclear circularity, disaggregation of nucleoli and a global loss of dense heterochromatin. Domains formed by repeat sequences when depleted of macroH2A are disorganized, expanded and fragmented, and partially re-expressed. This can be explained by our finding that macroH2A tethers these repeats to the nucleostructural protein Lamin B1. ChIPseq to determine the distribution of histone variants macroH2A1 and macroH2A2 were realized in the HepG2 cell line

ORGANISM(S): Homo sapiens  

SUBMITTER: Melanija Posavec  Julien Douet   Roberto Malinverni   Marcus Buschbeck    

PROVIDER: E-GEOD-58175 | ArrayExpress| 2016-06-04

REPOSITORIES: ArrayExpress

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