Transcriptomics

Dataset Information

297

ICLIP mapping of coilin-RNA interactions in HeLa and P19 cells


ABSTRACT: Our ChIP resuls suggested that coilin association with U3, snRNA and histone genes might be dependent on coilin-RNA interaction. We used iCLIP of coilin-GFP expressed in HeLa and P19 cell lines at endogenous levels to identify coilin RNA targets and investigate RNA-binding specificity. P19 cells expressing GFP fused to a nuclear localization signal (GFP-NLS) was used as a negative control. iCLIP results revealed that coilin binds several classes of ncRNA including snRNAs, U3 snoRNA and scaRNAs. Interestlignly the majority of coilin targets were intronic snoRNAs, suggesting a novel role of CBs in snoRNA biogenesis. 5 biological replicates from P19 and 2 biological replicates from HeLa cells after UV-crosslinking. Negative control samples prepared from GFP-NLS fusion protein are stored uder accession E-MTAB-747.

ORGANISM(S): Musculus  

SUBMITTER: Martin Machyna   Karla Neugebauer  Karla M Neugebauer 

PROVIDER: E-GEOD-58448 | ArrayExpress | 2014-11-06

SECONDARY ACCESSION(S): GSE58448SRP043186PRJNA252666

REPOSITORIES: GEO, ArrayExpress, ENA

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