Genomics

Dataset Information

298

Genome-wide identification of CRISPR/Cas9 off-targets in human genome


ABSTRACT: By a robust unbiased ChIP-seq approach, we demonstrated that CRISPR/Cas9 had crRNA-specific off-target binding activities in human genome. However, most of those binding off-targets could not be efficiently cleaved both in vivo and in vitro which suggested the cleavage off-target activity of CRISPR/Cas9 in human genome is very limited. We provided a valuable tool to further investigate the molecular mechanism of CRISPR/Cas9 and to optimize its in vivo targeting sgRNA binding sites were identified with ChipSeq by using GFP antibody (there are 2 replicates for egfa-t1 sgRNA,emx1 sgRNA and control without sgRNA in Hek293T cells, one egfa-t1 sgRNA,emx1 sgRNA and control without sgRNA in HeLaS3 cells)

ORGANISM(S): Homo sapiens  

SUBMITTER: Guangqing Lu   xie zhan  Jinzhi Duan  Yu Zhang 

PROVIDER: E-GEOD-58511 | ArrayExpress | 2014-06-18

SECONDARY ACCESSION(S): SRP043321GSE58511PRJNA252938

REPOSITORIES: GEO, ArrayExpress, ENA

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