Transcriptomics

Dataset Information

2

Increased B52 expression does not affect affect global gene expression in Drosophila


ABSTRACT: Deciphering the role of alternative splicing in developmental processes relies on the identification of key genes whose expression is controlled by splicing regulators throughout growth of a whole organism. Targeting expression of five SR proteins in the developing eye of Drosophila allowed us to show that these splicing factors induce various phenotypic alterations concerning eye organogenesis and viability. Although both dASF/SF2 and B52 caused defects in ommatidia structure, only B52 impairs normal photoreceptor axons projection and neurogenesis in visual ganglia. Consistently, microarray analyses revealed that many of the B52 targets are involved in brain organogenesis and we show that their splicing profile is altered both in B52 loss and gain of function. Conversely, a large proportion of dASF/SF2 targets are involved in eye development. This differential effect argues that SR proteins confer accuracy to developmental gene-expression programs, thus ensuring tissue identity and supporting cell-lineage decisions. Keywords: genetic modification Experiment aimed at determining whether increased SR protein B52 expression can affect global gene expression in Drosophila. Control: GMR X GFP-NLS trangenic larvae The 2 samples correspond to dye-swap experiments.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Jamal Tazi   Johann Soret  Jerome Dejardin  johann Soret  Mathieu Gabut 

PROVIDER: E-GEOD-5855 | ArrayExpress | 2007-09-17

SECONDARY ACCESSION(S): GSE5855PRJNA104397

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

The SR family proteins B52 and dASF/SF2 modulate development of the Drosophila visual system by regulating specific RNA targets.

Gabut Mathieu M   Dejardin Jérôme J   Tazi Jamal J   Soret Johann J  

Molecular and cellular biology 20070205 8


Deciphering the role of alternative splicing in developmental processes relies on the identification of key genes whose expression is controlled by splicing regulators throughout the growth of a whole organism. Modulating the expression levels of five SR proteins in the developing eye of Drosophila melanogaster revealed that these splicing factors induce various phenotypic alterations in eye organogenesis and also affect viability. Although the SR proteins dASF/SF2 and B52 caused defects in omma  ...[more]

Similar Datasets

2007-09-18 | E-GEOD-5854 | ArrayExpress
2007-09-17 | E-GEOD-5858 | ArrayExpress
2007-09-17 | E-GEOD-5857 | ArrayExpress
2007-09-18 | GSE5854 | GEO
2007-09-18 | GSE5855 | GEO
2007-09-18 | GSE5858 | GEO
2007-09-18 | GSE5857 | GEO
2013-04-05 | E-GEOD-44591 | ArrayExpress
2014-11-23 | E-GEOD-63263 | ArrayExpress
2008-12-30 | E-GEOD-11491 | ArrayExpress