Genomics

Dataset Information

242

MethlyC-Seq of wild type (with 35S-SUC2 transgene) and idm3-1, mbd7-1 mutants (same 35S-SUC2 background)


ABSTRACT: In eukaryotes, heterochromatin is characterized by numerous epigenetic marks, including DNA methylation. Spreading of these marks into nearby active genes must be avoided in order to maintain appropriate gene expression. Here, we uncover Arabidopsis Methyl-CpG-Binding Domain 7 (MBD7) and Increased DNA Methylation 3 (IDM3) as anti-silencing factors that prevent transgene repression and genome-wide DNA hypermethylation. MBD7 preferentially binds to highly methylated, CG-dense regions associated with non-CG methylation and physically associates with other anti-silencing factors, including the histone acetyltransferase IDM1, IDM2, and IDM3. IDM1 and IDM2 were previously shown to facilitate active DNA demethylation by the 5-methylcytosine DNA glycosylase/lyase ROS1. Thus, MBD7 tethers the IDM proteins to methylated DNA, which enables the function of DNA demethylases that in turn establish chromatin boundaries and limit DNA methylation Using MethylC-Seq to provide single-base resolution of DNA methylation status in WT and idm3-1, mbd7-1 mutants Whole genome methylation maps of mbd7-1, idm3-1 and WT (all three are from 35S-SUC transgene background) were generated using BS-seq

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Mingguang Lei   Zhaobo Lang  Xingang Wang  Jian-Kang Zhu  Daisuke Miki  Kai Tang 

PROVIDER: E-GEOD-58787 | ArrayExpress | 2015-01-29

SECONDARY ACCESSION(S): GSE58787SRP043539PRJNA253489

REPOSITORIES: GEO, ArrayExpress, ENA

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