Transcriptomics

Dataset Information

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RNAseq data from in vitro-stimulated (6 hours) murine relfl/flCD19-Cre and CD19-Cre splenic B cells purified by magnetic cell separation (REL6)


ABSTRACT: RNAseq analysis of CD40 + IgM in vitro-stimulated (6 hours) murine relfl/flCD19-Cre (furtheron designated as REL) and CD19-Cre (furtheron designated as WT) splenic B cells identifies genes regulated by the transcription factor c-REL in activated B cells. Splenic B cells from 12-week old relfl/flCD19-Cre and CD19-Cre littermate mice were isolated by magnetic cell separation from splenic mononuclear cells and stimulated in vitro for 6 hours with anti-CD40 and anti-IgM. RNA was isolated and submitted for RNA-sequencing on an Illumina HiSeq2000 instrument for 30 million single-ended reads.

ORGANISM(S): Mus musculus  

SUBMITTER: Ulf Klein   Nilushi De Silva 

PROVIDER: E-GEOD-58971 | ArrayExpress | 2014-07-15

SECONDARY ACCESSION(S): GSE58971SRP043713PRJNA254038

REPOSITORIES: GEO, ArrayExpress, ENA

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