Transcriptomics

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Expression data from of HD-iPSC and CON-iPSC neuron derivatives


ABSTRACT: Compared the global gene expression profiles of HD- and CON-iPSC-derived neurons We used microarrays to detail the global programme of gene expression for comparing the global gene expression profiles of HD- and CON-iPSC-derived neurons and facilitating studies of medium spiny neurons (MSN)-degenerative processes of Huntington's Disease (HD). By using a step-wise in vitro differentiation protocol combining EB formation, neural induction by small molecules, treatment with inhibitors of the TGFß pathway (SB431542) and the BMP pathway (LDN193189), and mechanical isolation/purification of neural progenitors and neurons, we induced 60-70% of control iPSCs or HD-iPSCs to differentiate into GABA- and DARPP-32- double positive neurons.

ORGANISM(S): Homo sapiens  

SUBMITTER: Hung-Chih Kuo   Feng-Lan Chiu  Chun-Ying Yu 

PROVIDER: E-GEOD-59051 | ArrayExpress | 2015-09-02

SECONDARY ACCESSION(S): GSE59051PRJNA254211

REPOSITORIES: GEO, ArrayExpress

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Publications

Elucidating the role of the A2A adenosine receptor in neurodegeneration using neurons derived from Huntington's disease iPSCs.

Chiu Feng-Lan FL   Lin Jun-Tasi JT   Chuang Ching-Yu CY   Chien Ting T   Chen Chiung-Mei CM   Chen Kai-Hsiang KH   Hsiao Han-Yun HY   Lin Yow-Sien YS   Chern Yijuang Y   Kuo Hung-Chih HC  

Human molecular genetics 20150811 21


Huntington's disease (HD) is an autosomal-dominant degenerative disease caused by a cytosine-adenine-guanine trinucleotide expansion in the Huntingtin (htt) gene. The most vulnerable brain areas to mutant HTT-evoked toxicity are the striatum and cortex. In spite of the extensive efforts that have been devoted to the characterization of HD pathogenesis, no disease-modifying therapy for HD is currently available. The A2A adenosine receptor (A2AR) is widely distributed in the brain, with the highes  ...[more]

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