Genomics

Dataset Information

298

BAF250a regulates nucleosome occupancy and histone modification in priming embryonic stem cell differentiation


ABSTRACT: BAF complex is one major group of chromatin remodeling factors in mammals. However, how BAF regulated nucleosomes and other histone modifications is not clear. Here we delete BAF250a, a major component in esBAF to study the nucleosome and histone changes in ESCs. We find that deletion of BAF250a leads to nucleosome occupancy increase in TSS regions and non-pioneer transcription factor binding sites. BAF250a deletion also cause overall decrease of H3K27me3 modification. Collectively, these results reveals how BAF complex coordinates nucleosome, histone modification to control ESC function. Sample 1-4: Nucleosome profiles in WT and BAF250a KO ESCs. Sample 5-10: profiling of H3K4me3 and H3K27me3 in WT and BAF250 KO ESCs.

ORGANISM(S): Mus musculus  

SUBMITTER: Jason West   Zhong Wang  Peng Fang  Jonathan H Dennis  Ienglam Lei  Robert Kingston  Xiaolin Gao 

PROVIDER: E-GEOD-59082 | ArrayExpress | 2015-08-03

SECONDARY ACCESSION(S): GSE59082SRP044051PRJNA254343

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

BAF250a Protein Regulates Nucleosome Occupancy and Histone Modifications in Priming Embryonic Stem Cell Differentiation.

Lei Ienglam I   West Jason J   Yan Zhijiang Z   Gao Xiaolin X   Fang Peng P   Dennis Jonathan H JH   Gnatovskiy Leonid L   Wang Weidong W   Kingston Robert E RE   Wang Zhong Z  

The Journal of biological chemistry 20150612 31


The unique chromatin signature of ES cells is fundamental to the pluripotency and differentiation of ES cells. One key feature is the poised chromatin state of master developmental genes that are transcriptionally repressed in ES cells but ready to be activated in response to differentiation signals. Poised chromatin in ES cells contains both H3 Lys-4 trimethylation (H3K4me3) and H3 Lys-27 trimethylation (H3K27me3) methylation, indicating activating and repressing potential. However, the contrib  ...[more]

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