Project description:Transcriptional profiling of Deinococcus radiodurans comparing control untreated wild type cells with wild type cells treated with 100 µM CdCl2. Two-condition experiment, wild type vs. 100uM CdCl2. Biological replicates: 4 time courses (0.25, 0.5, 1, 2h), dye-swapped, independently grown and harvested. Two replicate per array.
Project description:Microarray analyses with cells/tissues overexpressing YAP have revealed many transcription targets of YAP (Dong et al, 2007; Zhao et al, 2008). However, as YAP induces transformation of non-cancerous cells, we thought many of known targets of YAP may be indirect consequence of transforming property of YAP. To identify the immediate transcription targets for YAP, we utilized immortalized mammary epithelial MCF-10A cells expressing a tamoxifen inducible, hyperactive (S127/381A) YAP mutant (MCF-10A ERT2-YAP 2SA). MCF-10A ERT2 and MCF-10A ERT2-YAP 2SA are generated. Each cell line was treated with 0.1% of ethanol (solvent) or 1uM of 4-hydroxytamoxifen for 2 or 6 hours. This makes 6 samples per set. The experiments were done in duplicate. The expression data from MCF-10A ERT2 and MCF-10A ERT2-YAP 2SA before tamoxifen treatment can serve as control.
Project description:Transcriptional profiling of comparing wildtype strains with DR0199-deletion strains under normal growth conditions. One-condition experiment, wild type strains vs. DR0199-deletion strains. Three biological replicates were carried out.
Project description:Transcriptional profiling of comparing wildtype strains with DR1790-deletion strains under normal growth contidions. Keywords: Genetic modification One-condition experiment, wild type strains vs. DR1790-deletion strains. Two biological replicates were carried out.
Project description:Transcriptional profiling of E. coli cells comparing control harboring the empty vector pRadGro (Ec-pR) with E. coli expressing the Deinococcus radiodurans response regulator DR1558 (Ec-1558) Expression of DR1558 conferred to multi-stress tolerance to E. coli. Cells grown to exponential phase (OD600 = 0.8) were harvested. Biological replicates, 3. Escherchia coli K12 oligonucleotide 3X15 K microarray (MYcroarray Inc. USA)