Transcriptomics

Dataset Information

3

Transcription profiling of human granulocytic differentiation of primary CD34+ cells cultured under 20% O2 levels


ABSTRACT: Little is known about the global transcriptional program underlying granulocytic (G) commitment, differentiation and maturation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of G differentiation of human CD34-positive hematopoietic stem and progenitor cells cultured with interleukin-3, interleukin-6 granulocyte colony stimulating factor and stem cell factor. The goal this study was to identify genes involved in the various facets of G differentiation including commitment, expansion, differentiation and functional capacity. Experiment Overall Design: G-CSF mobilized peripheral blood CD34-positive cells were cultured with IL-3, IL-6, SCF and G-CSF under 20% O2. Three biological replicate experiments were analyzed and approximately 15% of the samples were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference RNA pool labeled with Cy5.

ORGANISM(S): Homo sapiens  

SUBMITTER: Eleftherios T. Papoutsakis  

PROVIDER: E-GEOD-5917 | ArrayExpress | 2008-06-14

SECONDARY ACCESSION(S): GSE5917PRJNA104409

REPOSITORIES: GEO, ArrayExpress

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