Genomics

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Genomic landscape of Epstein-Barr virus nuclear antigen 3A


ABSTRACT: We undertook ChIP-Seq of HA-tagged EBNA3A in Lymphoblastoid Cell Lines to understand the effects of this essential viral transcription factor on the cell DNA. We discovered that EBNA3A bound to DNA with BATF, IRF4 and RUNX3, making these Transcription Factors the ones that tether EBNA3A to DNA, allowing it to mediate its downstream effects. ChIP-Seq of 2 biological replicates of HA-tagged EBNA3A were performed. Input was obtained by reverse-crosslinking the cross-linked DNA

ORGANISM(S): Homo sapiens  

SUBMITTER: Sizun Jiang  Bradford Willox   Eric Johannsen   Elliott Kieff   Hufeng Zhou   Stefanie C Schmidt   Bo Zhao   Amy M Holthaus   Peter Kharchenko    

PROVIDER: E-GEOD-59181 | ArrayExpress | 2015-01-06

SECONDARY ACCESSION(S): GSE59181PRJNA254544

REPOSITORIES: GEO, ArrayExpress

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Publications

Epstein-Barr virus nuclear antigen 3A partially coincides with EBNA3C genome-wide and is tethered to DNA through BATF complexes.

Schmidt Stefanie C S SC   Jiang Sizun S   Zhou Hufeng H   Willox Bradford B   Holthaus Amy M AM   Kharchenko Peter V PV   Johannsen Eric C EC   Kieff Elliott E   Zhao Bo B  

Proceedings of the National Academy of Sciences of the United States of America 20141224 2


Epstein-Barr Virus (EBV) conversion of B-lymphocytes to Lymphoblastoid Cell Lines (LCLs) requires four EBV nuclear antigen (EBNA) oncoproteins: EBNA2, EBNALP, EBNA3A, and EBNA3C. EBNA2 and EBNALP associate with EBV and cell enhancers, up-regulate the EBNA promoter, MYC, and EBV Latent infection Membrane Proteins (LMPs), which up-regulate BCL2 to protect EBV-infected B-cells from MYC proliferation-induced cell death. LCL proliferation induces p16(INK4A) and p14(ARF)-mediated cell senescence. EBNA  ...[more]

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