Transcriptomics

Dataset Information

6

Fibronectin regulates Wnt7a signaling and satellite cell expansion


ABSTRACT: The influence of the extracellular matrix (ECM) within the stem cell niche remains poorly understood. We found that Syndecan-4 (Sdc4) and Frizzled-7 (Fzd7) form a coreceptor complex in satellite cells and that binding of the ECM glycoprotein Fibronectin (FN) to Sdc4 stimulates the ability of Wnt7a to induce the symmetric expansion of satellite stem cells. Newly activated satellite cells dynamically remodel their niche via transient high-level expression of FN. Knockdown of FN in prospectively isolated satellite cells severely impaired their ability to repopulate the satellite cell niche. Conversely, in vivo overexpression of FN with Wnt7a dramatically stimulated the expansion of satellite stem cells in regenerating muscle. Therefore, activating satellite cells remodel their niche through autologous expression of FN that provides feedback to stimulate Wnt7a signaling through the Fzd7/Sdc4 coreceptor complex. Thus, FN and Wnt7a together regulate the homeostatic levels of satellite stem cells and satellite myogenic cells during regenerative myogenesis. The data set contains one microarray of pooled quiescent skeletal muscle satellite cells

REANALYSIS of: GSE24811GSE24904

ORGANISM(S): Mus musculus  

SUBMITTER: Michael A Rudnicki   Florian Bentzinger  Carol Perez-Iratxeta 

PROVIDER: E-GEOD-59272 | ArrayExpress | 2014-07-11

SECONDARY ACCESSION(S): GSE59272PRJNA255003

REPOSITORIES: GEO, ArrayExpress

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Publications

Fibronectin regulates Wnt7a signaling and satellite cell expansion.

Bentzinger C Florian CF   Wang Yu Xin YX   von Maltzahn Julia J   Soleimani Vahab D VD   Yin Hang H   Rudnicki Michael A MA  

Cell stem cell 20130101 1


The influence of the extracellular matrix (ECM) within the stem cell niche remains poorly understood. We found that Syndecan-4 (Sdc4) and Frizzled-7 (Fzd7) form a coreceptor complex in satellite cells and that binding of the ECM glycoprotein Fibronectin (FN) to Sdc4 stimulates the ability of Wnt7a to induce the symmetric expansion of satellite stem cells. Newly activated satellite cells dynamically remodel their niche via transient high-level expression of FN. Knockdown of FN in prospectively is  ...[more]

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