Transcriptomics

Dataset Information

299

The G Protein-coupled Receptor P2Y14 Influences Insulin Release and Smooth Muscle Function in Mice


ABSTRACT: UDP-sugars were identified as extracellular signaling molecules, assigning a new function to these compounds in addition to their well defined role in intracellular substrate metabolism and storage. Previously regarded as an orphan receptor, the G protein-coupled receptor (GPCR) P2Y14 (GPR105) was found to bind extracellular UDP and UDP-sugars. Little is known about the physiological functions of this GPCR. To study its physiological role we used a gene-deficient (KO) mouse strain expressing the bacterial LacZ reporter gene to monitor the physiological expression pattern of P2Y14. We found that P2Y14 is mainly expressed in pancreas and salivary glands and in subpopulations of smooth muscle cells of the gastrointestinal tract, blood vessels, lung and uterus. Among other phenotypical differences KO mice showed a significantly impaired glucose tolerance following oral and intraperitoneal glucose application. An unchanged insulin tolerance suggested altered pancreatic islet function. Transcriptome analysis of pancreatic islets showed that P2Y14 deficiency significantly changed expression of components involved in insulin secretion. Insulin secretion tests revealed a reduced insulin release from P2Y14-deficient islets highlighting P2Y14 as a new modulator of proper insulin secretion. 10 samples from pancreatic islets isolated from wildtype mice; 10 samples from pancreatic islets isolated from P2Y14-knockout mice

ORGANISM(S): Mus musculus  

SUBMITTER: Johannes Grosse   Albert Ricken  Tobias Polte  Jaroslawna Meister  Torsten Schoneberg  Ralph Burkhardt  Torsten Schöneberg  Diana Le Duc  Angela Schulz  Helga Pfannkuche  Joachim Thiery 

PROVIDER: E-GEOD-59285 | ArrayExpress | 2014-07-11

SECONDARY ACCESSION(S): SRP044222GSE59285PRJNA254968

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

The G protein-coupled receptor P2Y14 influences insulin release and smooth muscle function in mice.

Meister Jaroslawna J   Le Duc Diana D   Ricken Albert A   Burkhardt Ralph R   Thiery Joachim J   Pfannkuche Helga H   Polte Tobias T   Grosse Johannes J   Schöneberg Torsten T   Schulz Angela A  

The Journal of biological chemistry 20140703 34


UDP sugars were identified as extracellular signaling molecules, assigning a new function to these compounds in addition to their well defined role in intracellular substrate metabolism and storage. Previously regarded as an orphan receptor, the G protein-coupled receptor P2Y14 (GPR105) was found to bind extracellular UDP and UDP sugars. Little is known about the physiological functions of this G protein-coupled receptor. To study its physiological role, we used a gene-deficient mouse strain exp  ...[more]

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