Transcriptomics

Dataset Information

61

Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells


ABSTRACT: To characterize Klf4 downstream genes, we performed microarray analyses of the ES cells knocked down by siRNA of Klf4 gene. Keywords: individual genetic characteristic design ES cells grow without feeders on gelatin coated in complete ES medium. Cells were incubated at 37 0C; 5% CO2. ES cells were transfected with siRNA and pCAGEGFP-IP plasmid. After transfection, cells were cultured for 24 hours, following to drag selection for more 24 hours in the presence of puromycin to ensure concentration of transfectants. Total RNA were extracted with Trizol reagent (Sigma) according to the manufacturer’s instructions.

ORGANISM(S): Mus musculus  

SUBMITTER: Minoru S.H. Ko   Nobutaka Fukui  Kadue Takahashi  Shinji Masui  Yuko Iwamatsu  Hitoshi Niwa  Jun-ichi Miyazaki  Kiyohito Yagi  Minoru S Ko  Rika Yagi  Ryo Matoba  Yuhki Nakatake 

PROVIDER: E-GEOD-5930 | ArrayExpress | 2010-06-10

SECONDARY ACCESSION(S): GSE5930PRJNA97379

REPOSITORIES: GEO, ArrayExpress

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Publications

Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells.

Nakatake Yuhki Y   Fukui Nobutaka N   Iwamatsu Yuko Y   Masui Shinji S   Takahashi Kadue K   Yagi Rika R   Yagi Kiyohito K   Miyazaki Jun-Ichi J   Matoba Ryo R   Ko Minoru S H MS   Niwa Hitoshi H  

Molecular and cellular biology 20060905 20


Although the POU transcription factor Oct3/4 is pivotal in maintaining self renewal of embryonic stem (ES) cells, little is known of its molecular mechanisms. We previously reported that the N-terminal transactivation domain of Oct3/4 is required for activation of Lefty1 expression (H. Niwa, S. Masui, I. Chambers, A. G. Smith, and J. Miyazaki, Mol. Cell. Biol. 22:1526-1536, 2002). Here we test whether Lefty1 is a direct target of Oct3/4. We identified an ES cell-specific enhancer upstream of the  ...[more]

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