Expression of miRNA induced by SDF-1/CXCR4 system on oral cancer
ABSTRACT: We examined the metastasis-related miRNAs induced by SDF-1/CXCR4 system, using oral cancer cells. Consequently, we identified 4 kinds of upregulated-miRNAs in B88-SDF-1. The metastasis-related miRNAs induced by SDF-1/CXCR4 system in oral cancer are largely unknown. Thus, we examined the metastasis-related miRNAs induced by SDF-1/CXCR4 system, using four types of oral cancer cells; mock cells vs forced-expression of SDF-1cells and parental cells vs parental cells treated by SDF-1.
Project description:Robust genes were up-regulated in the oral cancer cells with SDF-1/CXCR4 system; however, most of the genes did not exhibit metastasis-related functions. Mock cells and B88-SDF-1 cells, which have an autocrine SDF-1/CXCR4 system and exhibit distant metastatic potential in vivo were used.
Project description:We compared the profile of miRNAs expressed in HEK293 and MRC5 cells that overexpressed KRASG12V to those expressed in parental cells that harbored wild-type KRAS. The results indicated that a subset of miRNAs was significantly down-regulated in KRASG12V transfected two cells. To address the functional relevance of miRNAs in KRAS mutant cancers, we transfected exogenous KRASG12V into HEK293 and MRC5 cells with wild type KRAS genes, and we comprehensively profiled the dysregulated miRNAs.
Project description:The Epstein-Barr virus (EBV) encodes its own microRNAs (miRNAs); however, their biological roles remain elusive. The commonly used EBV B95-8 strain lacks a 12 kb genomic region, known as BamHI A Rightward Transcripts (BART) locus, which encodes a number of viral miRNAs (BART miRNAs). These miRNAs are expressed abundantly in EBV-positive epithelial malignancies, suggesting that the 12 kb region somehow contributes to EBV-mediated epithelial carcinogenesis. Bacterial artificial chromosome (BAC) technology was used to generate an EBV B95-8 strain in which the 12 kb region was fully restored at its native locus (BART-restored virus). HEK293 and AdAH cells infected with either the parental BART-deleted virus or the BART-restored virus were established. The gene expression profiles of these cells were examined to identify cellular target genes of BART miRNAs. Total RNAs of 2 independent v-miRNA-positive HEK293 (or AdAH) cells and 2 independent v-miRNA-negative cells HEK293 (or AdAH) cells were processed for Microarray analysis using 3D-Gene human Oligo chip 25k (Toray, Tokyo, Japan).
Project description:To elucidate the aetiology of HEH, especially with regard to microRNA (miRNA) profiles, samples obtained from three different parts of both normal and tumour tissues were analysed . The details of RNA extraction have been described in our previous reports. Interestingly, 16 miRNAs were significantly up-regulated and 51 miRNAs were down-regulated in the tumour tissues compared to the normal tissues (Student’s t-test, p<0.01). In addition, unsupervised hierarchical clustering analysis using a Pearson’s correlation showed that the tumour tissues clustered separately from the normal tissues. microRNA samples obtained from three different parts of both normal and tumour tissues were analysed
Project description:miRNA expression profiling between GIST and leiomyoma specimens taken by Tunneling Bloc Biopsy Nine GIST patients and seven gastric leiomyoma patients underwent endoscopic biopsy called Tunnel Block Biopsy. MiRNAs were extracted from the tissues, then.
Project description:The miRNAs expression was markedly altered with the treatment of gemcitabine on human CCC cells in vitro. And various miRNAs induced by gemcitabine may contribute to tumor regression in vitro. Using a custom microarray platform, we analyzed the expression levels of 2555 human miRNA probes in the cell lines in vitro that were treated with and without gemcitabine.
Project description:Recent studies have demonstrated that micro (mi)RNA molecules can be detected in the circulation and can serve as potential biomarkers of various diseases. This study used microarray analysis to identify aberrantly expressed circulating miRNAs in patients with type 1 autoimmune hepatitis (AIH) compared with healthy controls. Patients with well-documented and untreated AIH were selected from the National Hospital Organization (NHO)-AIH-liver-network database. They underwent blood sampling and liver biopsy with inflammation grading and fibrosis staging before receiving treatment. To further confirm the microarray data, circulating expression levels of miR-21 and miR-122 were quantified by real-time quantitative polymerase chain reaction in 46 AIH patients, 40 patients with chronic hepatitis C (CHC), and 15 healthy controls. Consistent with the microarray data, serum levels of miR-21 were significantly elevated in AIH patients compared with CHC patients and healthy controls. miR-21 and miR-122 serum levels correlated with alanine aminotransferase levels. Circulating levels of miR-21 and miR-122 were significantly reduced in AIH patients with liver cirrhosis, and were inversely correlated with increased stages of fibrosis. By contrast, levels of circulating miR-21 showed a significant correlation with the histological grades of inflammation in AIH. We postulate that aberrantly expressed serum miRNAs are potential biomarkers of AIH and could be implicated in AIH pathogenesis. Alternations of miR-21 and miR-122 serum levels could reflect their putative roles in the mediation of inflammatory processes in AIH. Case-control study, steroid treatment
Project description:The miRNAs expression was markedly altered with the treatment of cisplatin in vitro and various miRNAs induced by cisplatin also may contribute to tumor growth in vitro. Using a custom microarray platform, we analyzed the expression levels of 1719 human miRNA probes in the cell lines in vitro that were treated with and without cisplatin.
Project description:The miRNAs expression was markedly altered with the treatment of gemcitabine on human CCC cells in vitro. And various miRNAs induced by gemcitabine may contribute to tumor regression in vitro . Using a custom microarray platform, we analyzed the expression levels of 1042 human miRNA probes in the cell lines in vitro that were treated with and without gemcitabine.
Project description:The miRNAs expression was markedly altered with the treatment of metformin in vivo and various miRNAs induced by metformin also may contribute to tumor growth of cholangiocarcinoma in vivo. Using a custom microarray platform, we analyzed the expression levels of 985 human miRNA probes in the tumorous tissues in vivo that was treated with and without metformin.