Genomics

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Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma [ChIP-seq]


ABSTRACT: ChIP-Seq analysis of a pediatric human diffuse intrinsic pontine glioma (DIPG) cell line SF8628, harboring the K27M mutation. Goal was to obtain quantitative estimates of K27me3 immunoprecipitation change between vehicle-treated SF8628 cells [dimethyl sulfoxide, (DMSO)] and SF8628 cells incubated with 6 mM GSKJ4 at 24 hours and 72 hours. Three-condition experiment: 72 h GSKJ4-treated cells vs. 24 h GSKJ4-treated cells vs. DMSO-treated cells. Biological replicates: 1 cell line, each with 2 technical replicates per condition.

ORGANISM(S): Homo sapiens  

SUBMITTER: Xi Huang   Vy Ngo  Xiaoyue Chen  Robin Lerner  Nalin Gupta  Claudia Petritsch  Noemi Andor  Sabine Mueller  Zhiguo Zhang  Pamela Paris  Dong Fang  Yuichiro Ihara  Todd Waldman  C D James  Rintaro Hashizume  David Solomon  Haiyun Gan  Maxwell W Tom 

PROVIDER: E-GEOD-59496 | ArrayExpress | 2014-07-20

SECONDARY ACCESSION(S): GSE59496SRP044382PRJNA255430

REPOSITORIES: GEO, ArrayExpress, ENA

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